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Simmler, Charlotte, Fronczek, Frank R., Pauli, Guido F., Santarsiero, Bernard D. (2013) Absolute configuration of naturally occurring glabridin. Acta Crystallographica Section C Crystal Structure Communications, 69 (11) 1212-1216 doi:10.1107/s0108270113018842

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Reference TypeJournal (article/letter/editorial)
TitleAbsolute configuration of naturally occurring glabridin
JournalActa Crystallographica Section C Crystal Structure Communications
AuthorsSimmler, CharlotteAuthor
Fronczek, Frank R.Author
Pauli, Guido F.Author
Santarsiero, Bernard D.Author
Year2013 (November 15)Volume69
Issue11
PublisherInternational Union of Crystallography (IUCr)
DOIdoi:10.1107/s0108270113018842Search in ResearchGate
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Mindat Ref. ID191237Long-form Identifiermindat:1:5:191237:5
GUID0
Full ReferenceSimmler, Charlotte, Fronczek, Frank R., Pauli, Guido F., Santarsiero, Bernard D. (2013) Absolute configuration of naturally occurring glabridin. Acta Crystallographica Section C Crystal Structure Communications, 69 (11) 1212-1216 doi:10.1107/s0108270113018842
Plain TextSimmler, Charlotte, Fronczek, Frank R., Pauli, Guido F., Santarsiero, Bernard D. (2013) Absolute configuration of naturally occurring glabridin. Acta Crystallographica Section C Crystal Structure Communications, 69 (11) 1212-1216 doi:10.1107/s0108270113018842
In(2013, November) Acta Crystallographica Section C Crystal Structure Communications Vol. 69 (11) International Union of Crystallography (IUCr)
Abstract/NotesThe title compound {systematic name: 4-[(3R)-8,8-dimethyl-3,4-dihydro-2H-pyrano[2,3-f]chromen-3-yl]benzene-1,3-diol, commonly named glabridin}, C20H20O4, is a species-specific biomarker from the rootsGlycyrrhiza glabraL. (European licorice, Fabaceae). In the present study, this prenylated isoflavan has been purified from an enriched CHCl3fraction of the extract of the root, using three steps of medium-pressure liquid chromatography (MPLC) by employing HW-40F, Sephadex LH-20 and LiChroCN as adsorbents. Pure glabridin was crystallized from an MeOH–H2O mixture (95:5 v/v) to yield colorless crystals containing one molecule per asymmetric unit (Z′ = 1) in the space groupP212121. Although the crystal structure has been reported before, the determination of the absolute configuration remained uncertain. Stereochemical analysis, including circular dichroism, NMR data and an X-ray diffraction data set with Bijvoet differences, confirms that glabridin, purified from its natural source, is found only in a C3Rconfiguration. These results can therefore be used as a reference for the assignment of the configuration and enantiopurity of any isolated or synthetic glabridin sample.


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